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Inventi Impact: Pharm Analysis & Quality Assurance

Articles

  • Inventi:ppaqa/30032/19
    An LC-MS/MS Method to Measure S-Methyl-L-Cysteine and S-Methyl-L-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards
    Research 2019 : October - December
    Tharsini Sivapalan, Antonietta Melchini, Jack Coode-Bate, Paul W Needs, Richard F Mithen, Shikha Saha

    This is the first report describing an analytical method for quantitative analysis of\ntwo naturally occurring sulphur compounds, S-methyl-l-cysteine (SMC) and S-methyl-l-cysteine\nsulfoxide (SMCSO), in human body fluids using isotope-labelled internal standards and liquid\nchromatography-mass spectrometry (LC-MS)/MS techniques. This method was validated according\nto the guideline of the Royal Society of Chemistry Analytical Methods Committee. It offers significant\nadvantages including simple and fast preparation of human biological samples. The limits of detection\nof SMC were 0.08 microM for urine and 0.04 microM for plasma. The limits of detection of SMCSO were\n0.03 microM for urine and 0.02 microM for plasma. The calibration curves of all matrices showed linearity\nwith correlation coefficients r2 > 0.9987. The intra and inter day precisions in three levels of known\nconcentrations were >10% and >20%, respectively.............................

    How to Cite this Article
    Attribution/ CC Compliant Citation: Sivapalan, Tharsini, et al. \"An LC-MS/MS Method to Measure S-Methyl-l-Cysteine and S-Methyl-l-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards.\" Molecules 24.13 (2019): 2427.\nhttps://doi:10.3390/molecules24132427\nhttp://creativecommons.org/licenses/by/4.0/\n\nSome formatting elements, header, footer, logos, dates and pagination were modified while adapting this article.
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